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Spontaneous mutations in the TCR and BCR genes are responsible for autoimmune diseases including several forms of arthritis. At least 11 disease-associated mutations have been identified in the variable (V), joining (J), and constant (C) regions of TCR and BCR genes in mice (Schuler K F. Autoimmunity: the key to understanding and treating autoimmune diseases. Nat Rev Immunol. 2006 March; 6(3):214-21). To correct disease-causing mutations, exogenous rearranged TCR and BCR genes have been inserted into the endogenous loci by ZFN and TALEN to reconstitute a functional TCR and BCR repertoire. While the therapeutic potential of site-specific DNA cleavage using ZFNs or TALENs is rapidly emerging, large (kb) rearrangements that often accompany these approaches remain a major challenge. Long range chromosomal DNA translocations causing pathogenic recombinations are a key source of genetic instability in cancer. Using Cas9/sgRNA-based approaches, it has been shown that Cas9 nuclease can mediate site-specific single or double strand DNA cleavage in vitro, in Xenopus, and in cultured human cells (Hsu et al., Cell. 2011 Feb. 28; 144(2):268-79). It was recently reported that in human cancer cells, Cas9 can be directed to recognize and cleave DNA at desired sites in the genome (Boch et al., Science 2013 April; 341(6149):837-40). In most of these cases, Cas9/sgRNA was reported to mediate double strand breaks, which were primarily repaired by non-homologous end joining (NHEJ) resulting in small DNA insertions or deletions. However, unlike the classical NHEJ pathway, the mechanisms associated with Cas9-directed mutagenesis or rearrangement events remain largely unknown. Furthermore, there has been no report on Cas9-mediated targeted genomic recombination in cell types other than cancer cells or on the restoration of a functional TCR repertoire in a relevant mouse disease model. 65a90a948d

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Metti, una sera a cena nasce come pièce teatrale, che Patroni Griffi porta in scena all'Eliseo di Roma nel 1967 con grande successo di pubblico e con repliche che si susseguono per ben due anni. Protagonisti della prima edizione, che aveva come regista Giorgio De Lullo, erano gli attori della Compagnia dei giovani: Romolo Valli, Rossella Falk, Elsa Albani e Carlo Giuffré, con Umberto Orsini nella parte di Ric.[1]
Pur trattandosi d'un film "d'autore", Metti, una sera a cena all'epoca fu uno dei campioni d'incassi della stagione 1968-69,[4] grazie al cast che comprendeva una giovanissima Florinda Bolkan (scoperta proprio dalla produttrice Marina Cicogna) qui al suo primo ruolo da co-protagonista. Il regista volle farla recitare nella famosa scena d'amore a tre, che pur provocando una serie di noie con la censura, fece entrare di diritto il film nell'allora emergente filone erotico attirando un grande numero di spettatori. In penombra, Max apre lentamente i bottoni del vestito della donna e poi la offre all'amico: con una sola immagine, quella delle tre mani intrecciate e tese, il regista creò una forte carica sensuale. L'intento di Patroni Griffi non era tanto quello di scandalizzare, quanto di disegnare un ritratto lucido e disincantato di un certo ambiente borghese, amorale e nevrotico.[5]
Un ruolo importante nel successo del film ebbe anche la colonna sonora, firmata da Ennio Morricone e diretta da Bruno Nicolai. Con una sequenza ascendente di sole tre note, ripetute ossessivamente lungo tutto il dipanarsi del tema musicale principale omonimo, Morricone costruì una bossa nova che poi divenne famosa anche fuori dal film, fu eseguita da numerose orchestre, gruppi musicali e anche cantanti solisti (con l'aggiunta di un testo dello stesso Patroni Griffi in inglese, con il titolo Hurry to Me, o italiano, con il titolo di Metti, una sera a cena), e ancora oggi è un classico della musica lounge.
Nello stesso 1969 Florinda Bolkan incise il 45 giri Metti, una sera a cena/Oggi te ne vai, pubblicato dalla DET Recording. Il disco, che contiene il tema Metti, una sera a cena, nella colonna sonora del film presente nella versione con vocalizzi di Edda Dell'Orso e, cantato in inglese, con il titolo di Hurry to Me, è qui cantato in italiano su testo dello stesso Giuseppe Patroni Griffi. Il singolo non fa tuttavia parte della colonna sonora originale del film.
Uscito nell'aprile 1969, Metti, una sera a cena si collocò subito al primo posto fra gli incassi mensili[10] e alla fine della stagione risultò tredicesimo nella classifica 1968-69 delle pellicole più viste.[4]
L'album contiene la colonna sonora del film Metti, una sera a cena, tratto dalla commedia omonima, diretto da Giuseppe Patroni Griffi e interpretato da Florinda Bolkan, Tony Musante, Jean-Louis Trintignant, Lino Capolicchio e Annie Girardot. Le musiche, composte e arrangiate da Ennio Morricone, sono interpretate dall'orchestra diretta da Bruno Nicolai.[3] Il brano Hurry to Me, ovvero il tema Metti, una sera a cena riarrangiato, contiene inoltre un testo in inglese, scritto dallo stesso Giuseppe Patroni Griffi e cantato da I Cantori Moderni di Alessandro Alessandroni.[3][4] Questo brano, inedito nell'edizione italiana del disco, è stato pubblicato come traccia bonus in edizioni successive, a partire da quella destinata al mercato britannico del 1970[3] ed è stato inoltre cantato in italiano da Florinda Bolkan nel 45 giri Metti, una sera a cena/Oggi te ne vai, che non fa tuttavia parte della colonna sonora ufficiale del film. Gli effetti speciali sono eseguiti dallo stesso Bruno Nicolai all'organo Thomas.[3] Edda Dell'Orso presta la voce nei vocalizzi presenti nelle tracce Metti, una sera a cena e Uno che grida amore.[3]
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