MEMO210308

MEMO210602

performed at Diagonale4 - Transfinity

All text passages are taken from the book To Live and Think Like Pigs - The Incitement of Envy and Boredom in Market Democracies
by Gilles Chatelet (translated by Robin Mackay)

70%gradient

Gliss String Synthesis

Helsinki

The yellow PCR product was cloned into the pCRII-TOPO vector (Invitrogen, San Diego), and the yellow probe was made using SP6 polymerase. apMM-Mcp was balanced over CyO, P{w[+mC]=ActGFP}JMR1 (BL#4533) to select homozygous apMM-Mcp larvae. Homozygous apMM-Mcp or apMM larvae were selected and the imaginal discs and central nervous system (CNS) were dissected in PBS. Tissues were fixed in 4% formaldehyde in PBS for 20 min at room temperature, while rocking. The tissues were then washed thoroughly with phosphate-buffered saline + 0.1% Triton X-100 (PBST) and allowed to prehybridize in hybridization buffer (50% formamide, 5× SSC, 50 μg/ml heparin, 0.1% Tween 20, 100 μg/ml sonicated salmon sperm DNA) for 2 hr at 55°. The DIG-labeled probes were then diluted 1:100, heated to 80°, added to the tissue, and incubated at 55° overnight to hybridize. The probe was then removed and the sample was washed extensively with hybridization buffer, followed by PBST. The sample was then probed with 1:2000 HRP-conjugated anti-DIG antibody (Roche) for 1.5 hr. Upon removal of the antibody, the sample was washed extensively with PBST and then washed twice with developing solution (0.1 m NaCl, 0.1 m Tris–HCl, pH 9.0, 0.05 m MgCl2, 0.1% Tween 20). The tissues in developing solution were transferred to a glass dish and 20 μl of solution [18.75 mg/ml Nitro blue tetrazolium chloride, 9.4 mg/ml 5-bromo-4-chloro-3′-indolyl phosphate, toluidine salt, in 67% dimethyl sulfoxide (w/v) (Roche)] was added. In situs were developed for between 30 and 60 min. The reaction was stopped by washing twice with PBST. Imaginal discs and brains were then mounted on slides in 70% glycerol and pictures were taken using a Nikon DXM200F digital camera on a Nikon Microphot-SA light microscope.

Transient Certitude

This piece was performed on the Blurred Edges Festival 2017 in Hamburg, Germany.

The audio file is the one that has been played to the audience and not the capturing of the concert situation.

 full text here:

https://ia601504.us.archive.org/34/items/AnachronisticDomain-Study1/Anachronistic%20Domain%20%E2%80%93%20Study%201.pdf

Anachronistic Domain Study 1 - Blurred Edges

Benjamin Flesser @West Germany 28.06.2018

Benjamin Flesser @Experimentik 20.03.2019

Electromagnetic Fields - Sicily

Brain activity controlling the modular synth. Stereo recording of quadrophonic performance by Adam Asnan.

https://www.musicasanae.com/berlin

benjamin flesser

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