Para Djs, Balade, Ben Bazen, Alt-J ∆, David Tossell, 8/9/22, Anni 90, Ey, Başı, Nergihan Yeşilyurt, Adversários, Desiree, 2019,Guaracha, Albert Heijn, Acidhouse, Airband, Chantiers D'été, 2021хит,, Alkaline Maniac, Alternativerockplaylist2020, Ajdapekkanyakargecerimremix, Blues,, Ankabut, 8, Atgdjs, Adoration,Louanges, Dietrolelinee, Clement, Analogue Games,  Amione,, Fractal, Ballsinyajawz, Guniguni, Coeo, 7220, Arbeiderbevegelsen, Jason Mraz, Cjr

Benz Dealer free background music for youtube 💀FREE DOWNLOAD

The final episode of our Organoids Miniseries is now live! In this episode, sponsored by Molecular Devices, we discuss the processes involved in scaling up three-dimensional (3D) organoids. 

This conversation features Dr Vicky Marsh Durban, Chief Executive Officer at Cellesce Limited, and Dr Magdalena Kasendra, who is the Director of Research and Development at Centre for Stem Cell and Organoid Medicine.

Key learning points:
●	Process of scaling up 3D high-throughput cell cultures 
●	Induced pluripotent stem cells (iPSCs) versus adult stem cells
●	Discover different kinds of solutions to overcome challenges related to scaling up 3D organoids.

Episode 18 – Scaling up organoids

1-5, -wohl-reichtum, -x plus dur-x divertissement, -------- audio - - - - lista de reproducción - - - -, ---- , morgenshtern, djbengals vs chiefs dj alanik - - -, película, ambiente scuro ambiente scuro musica di sottofondo ambiente scuro beat ambiente scuro drone, alnadi, allumeur plus léger,, amor, amor, música de amor, música, hermosa, anirémianirémix, --s filme- s musik머니, ---- trance gesangstrance ---- falle, ----?taxe - - - - a, ---- rnb - - - - roccia, 2015年、kfewenkpex, alan gomez nègre, mélange de lait chaud, 2021,scott,drake,trap, aerosmith gehen sie diesen wegaerosmith,, a cobra vai subir, afro love, ---- detroit - - - - deutschrap, 2015年ディベラグ-ヴィデン, ----,elvin ----,elvinklassischer film, agipagipcio, 3階録音ビット、リガー、ロックスフル第3世代ゲート第3世代, adub - - - adubblz, ---- griego - - - greekmix, 2015-0--13 20: 00: 00 pm-x-t-idz-dit, a halottak hadserege netflix film reviewarmy of the dead review, aperçus contractuels de l'industrie, acido pienico acido pienico, piensas piensasenmi, -----------사디피아노사디피아티피아티피아티피아티피아티피아티피아티피, ascending, americanpraisecore, ---- der ventilator ---- der junge laroi-typ beat, 2015-0--13 20: 00: 00 pm-10: 00 pm-10: 00 pm-10: 00 pm-10: 00 pm-10: 00 pm-10: 00 pm-10: 00 pm-10: 00 pm-10: 00 pm -, adhäsionsmadhoora millanممانچه, ---zoll,---instrumental, ----,eminem----,emotional, 200 bpm , album acoustique de lil mosey, aegé, 1- kasım 201- 11, gangnam-daero 133-gil, gangnam-gu, seul özel şehri (gangnam-dong, gangnam-dong,gangnam-dong,gangnam-dong,gangnam-dong,gangnam-dong,gangnam-dong, gangnam-dong, gangnam-dong, gangnam-dong, gangnam-dong, gangnam-dong, gangnam-dong, gangnam-dong, gangnam-dong), 2015-0--13 20: 00: 00 pm-andare con il gioco-flusso, aarhus, ---g_qsfp---h, allerleivonnicolai, -dmusic-dnl-, beat, emotionalbeat, συναισθηματική, λυπημένος, κιθάρα, ψάριbeat, energeticbeat, epic, action intro, accento, accentuatoinglese, alates-thefom:, 2023 mix, allayallayl'nın sohbeti, amitié-, -annajoyce___, a partir de hoy-mucho más, 100000000000 narukuchota, algumas horas, algumas coisas-alguns dias, --5

-瞑想-瞑想-瞑想-瞑想-瞑想-瞑想-瞑想-瞑想-瞑想-瞑想-瞑想-瞑想-瞑想-瞑想 corporate background music FREE DOWNLOAD

Bass boosted. From metal slug 3 final mission.

Metal Slug 3 Music- Bioinformatics (Mission Five Part Six)

Andrew, Lee, Torsten, and Fin muse over the future of MLST, Illumina, and Nanopore at the ASM NGS Hackathon

141 hackathon panel

Today we are talking about bioinformatics with Dr. Abel Torres Espin. Abel is an Assistant Professor and Director of the health data DRIVEN lab at the School of Public Health Sciences at the University of Waterloo in Canada. He has worked with...

More info: https://u2fp.org/get-educated/curecast/episode-125.html

SCI & AI (Episode 125)

I needed an old scifi sounding sample to use, so I grabbed a recording of my ramblings on the tree of life hypothesis at burning man...

Dr. 42 - Complicated Question

Giving reverb to music without reverb results in something amazing.

Metal Slug 3 - Bioinformatics with reverb

In this episode we identify areas of “Peak-bioinformatics”. There are a lot of existing bioinformatics software out there - more often than not the new tool you want to write already exists or a new tool cannot effectively improve. We discuss this in terms of genome assembly, read mapping and phylogenetics. 

Question and comments? microbinfie@gmail.com

SHOW NOTES
Generally, novel software is not needed if:
There are a plethora of existing tools
The problem is more or less solved or its been shown to be unsolvable
The underlying technology or problem is now obsolete and/or superceded by other methods. 

Multiple sequence aligners:
MAFFT https://mafft.cbrc.jp/alignment/software/
MUSCLE  https://www.drive5.com/muscle/ 
Whole genome aligners:
Mauve  http://darlinglab.org/mauve/mauve.html
Mugsy http://mugsy.sourceforge.net/
Sibellia  http://bioinf.spbau.ru/sibelia
Parsnp https://github.com/marbl/parsnp

Assemblers:
SPADES https://github.com/ablab/spades
Skesa  https://github.com/ncbi/SKESA
Velvet https://www.ebi.ac.uk/~zerbino/velvet/
Abyss https://github.com/bcgsc/abyss
Edena  http://www.genomic.ch/edena.php
Ray http://denovoassembler.sourceforge.net/

Long read assemblies
HGAP https://github.com/PacificBiosciences/Bioinformatics-Training/wiki/HGAP-2.0
Flye https://github.com/fenderglass/Flye
Canu https://github.com/marbl/canu
Ra https://www.biorxiv.org/content/10.1101/656306v1
Unicycler  https://github.com/rrwick/Unicycler

Read mapping
BWA http://bio-bwa.sourceforge.net/ 
Bowtie2 http://bio-bwa.sourceforge.net/
Minimap2 https://github.com/lh3/minimap2 (BWA better for short reads: https://lh3.github.io/2018/04/02/minimap2-and-the-future-of-bwa)
BBtools https://jgi.doe.gov/data-and-tools/bbtools/
BLAST/BLAT: https://genome.ucsc.edu/FAQ/FAQblat.html
SMALT https://www.sanger.ac.uk/science/tools/smalt-0
Snippy https://github.com/tseemann/snippy
Phenix:  https://github.com/phe-bioinformatics/PHEnix

Variant callers
GATK: https://software.broadinstitute.org/gatk/
VIPR: https://www.viprbrc.org/brc/home.spg?decorator=vipr  
Varscan2  http://varscan.sourceforge.net/

Workflow managers
Bespoke example https://github.com/VertebrateResequencing/vr-codebase
Snakemake. https://snakemake.readthedocs.io/en/stable/
Nextflow  https://www.nextflow.io/
Galaxy  https://usegalaxy.org/
Bpipe  https://github.com/ssadedin/bpipe

Phylogenetics:
Raxml - Raxml-NG https://cme.h-its.org/exelixis/software.html 
IQTREE http://www.iqtree.org/ 
FastTree http://www.microbesonline.org/fasttree/ 
BEAST 1&2 https://www.beast2.org/ 
RevBayes  https://revbayes.github.io/

Metagenomics
Taxonomic classification: Megan, Kraken, SIGMA, MIDAS, metaphlan2, mOTUs. 
Assemblers: MetaSpades, metaflye, MEGAHIT, MetaVelvet, a lot of single isolate assemblers have been tweaked to run on metagenomes.
https://github.com/lskatz/Kalamari

AMR
https://github.com/arpcard/amr_curation
https://food-safety-bioinformatics-hackathon.github.io/AMR-protocols/
ABRICATE https://github.com/tseemann/abricate 
ARIBA https://www.sanger.ac.uk/science/tools/ariba 
Too many detection tools:
https://docs.google.com/spreadsheets/d/18XGWpDiaE249qQKDAL7gdBCka0Z1drpA_s3FElfMJe0/edit#gid=0

Other mentioned resources
Mentioned Recent review. 
Zhang W, Chen J, Yang Y, Tang Y, Shang J, Shen B (2011) A Practical Comparison of De Novo Genome Assembly Software Tools for Next-Generation Sequencing Technologies. PLoS ONE 6(3): e17915. https://doi.org/10.1371/journal.pone.0017915
The Assemblerthon: https://assemblathon.org/
Blog post describing that BWA better for short reads: https://lh3.github.io/2018/04/02/minimap2-and-the-future-of-bwa
The science web: https://thescienceweb.wordpress.com/2015/03/23/each-bioinformatician-to-have-their-own-personal-short-read-aligner-by-2016/

01 What bioinformatics software not to write part 1

We chat to Justin O'Grady, Andrew Page and Alison Mather about how they went about sequencing 1500 SARS-CoV-2 genomes from one small region in the UK (Norfolk), and how they went about using the data for genomic epidemiology to help get a detailed, near real-time view of the pandemic as it unfolded.

Some of the key points are that in Norfolk and surrounding regions: 
100 distinct UK lineages were identified.
16 UK lineages found in key workers were not observed in patients or in community care. 
172 genomes from SARS-CoV-2 positive samples sequenced per 100,000 population representing 42.6% of all positive cases.
SARS-CoV-2 genomes from 1035 cases sequenced to a high quality.
Only 5 countries, out of 103, have sequenced more SARS-CoV-2 genomes than have been sequenced in Norfolk for this paper.
Samples covered the entire first wave, March to August 2020.
Stable evolutionary rate of 2 SNPs per month.
D614G mutation is the dominant genotype and associated with increased transmission.
No evidence of reinfection in 42 cases with longitudinal samples.
WGS identified a sublineage associated with care facilities.
WGS ruled out nosocomial outbreaks. 
Rapid WGS confirmed the relatedness of cases from an outbreak at a food processing facility.

The manuscript is available from: https://www.medrxiv.org/content/10.1101/2020.09.28.20201475v1

31 Large scale sequencing of SARS-CoV-2 genomes from one region

I Like Music. I Like Sandboxes. I Like Level Editors. I Like Psychotic Stuff. I Like, Eh... Videogames. I Like Good Pals. I Like Music.
 You Cant Return The Slab. Now Get Lost!

I'm scientist and DR42 is my persona who likes to make mashups and techno.

Unite 2 Fight Paralysis exists to unite and empower the international spinal cord injury community to cure paralysis through advocacy, education, and support for research. Get Educated and Get Involved.

Microbial Bioinformatics is a rapidly changing field marrying computer science and microbiology. Join us as we share some tips and tricks we’ve learnt over the years. If you’re student just getting to grips to the field, or someone who just wants to keep tabs on the latest and greatest - this podcast is for you. 

The hosts are Dr. Lee Katz from the Centres for Disease Control and Prevention (US), Dr. Nabil-Fareed Alikhan from the University of Oxford (UK), and Prof. Andrew Page from Theiagen Genomics (UK) and bring together years of experience in microbial bioinformatics.

The opinions expressed here are our own and do not necessarily reflect the views of Centres for Disease Control and Prevention, University of Oxford or Theiagen Genomics.

Intro music : Werq - Kevin MacLeod (incompetech.com)
Licensed under Creative Commons: By Attribution 3.0 License
http://creativecommons.org/licenses/by/3.0/

Outro music : Scheming Weasel (faster version) - Kevin MacLeod (incompetech.com)
Licensed under Creative Commons: By Attribution 3.0 License
http://creativecommons.org/licenses/by/3.0/

Question and comments? microbinfie@gmail.com

Popular tracks tagged #bioinformatics