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Biología celular y molecular, séptima edición, es una obra dirigida a todo estudiante del área biomédica, y está encaminada a mostrar conceptos fundamentales como la relación entre estructura molecular y función, el carácter dinámico de los organelos (orgánulos celulares), el sitio de energía química para la consecución de actividades celulares y asegurar la biosíntesis macromolecular adecuada a la unidad y diversidad simultáneas a niveles macromoleculares, y los mecanismos que regulan actividades celulares. Este texto ofrece al lector 18 capítulos y un glosario ilustrados a todo color donde se muestra a detalle los temas. Cada capítulo incluye las pruebas experimentales que justifican las conclusiones del mismo.En forma conjunta, la séptima edición contiene más de 100 nuevas micrografías e imágenes obtenidas por computadora, todas provenientes de la fuente original y revisadas con gran cuidado.Se incluyen comentarios breves del autor sobre experimentos selectos de gran importancia, para reforzar las bases experimentales en el conocimiento de la materia.Para estudiantes e instructores que deseaban explorar con mayor profundidad el enfoque experimental, se incluye la sección de Vías experimentales al final de muchos de los capítulos.Para los estudiantes del área de medicina y de pregrado, se incluye el apartado de Perspectiva humana, donde se ilustra que prácticamente todos los trastornos de los seres humanos se pueden atribuir a la perturbación de actividades a nivel celular y molecular.Incluye un centro de aprendizaje en línea.
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La infección por el parásito Entamoeba histolytica es causa de disentería y absceso hepático amebianos, enfermedades asociadas con alta morbi-mortalidad. En el caso de la amebiosis hepática, el papel de la inmunidad humoral en la protección no ha sido del todo establecido pero tiene importancia en estudios seroepidemiológicos y métodos diagnósticos. La inmunidad celular es importante para el control de la infección y desarrollo de inmunidad protectora luego de amebiosis invasiva. Las técnicas de genética molecular y los nuevos modelos de experimentación han enseñado mucho acerca de la patogénesis de la amebiosis, su entendimiento adecuado junto con el conocimiento de la biología molecular y genética del parásito y los mecanismos inmunes del huésped permitirá no sólo el desarrollo de nuevas opciones diagnósticas y de tratamiento sino también de una vacuna efectiva y segura para eliminar la enfermedad.
En los últimos años, las técnicas de genética molecular y los nuevos modelos de experimentación han enseñado mucho acerca de la patogénesis de la amebiosis, pero a pesar de su importancia, persisten interrogantes acerca de la respuesta inmune frente a la infección por este parásito (4). Hallazgos característicos en la inmunidad celular y humoral han sido detectados en pacientes con enfermedad invasiva. 7ad9723583

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The biological world operates on a multitude of scales - from molecules to tissues to organisms to ecosystems. Throughout these myriad levels runs a common thread: the communication and onward passage of information, from cell to cell, from organism to organism and ultimately, from generation to generation. But how does this information come alive to govern the processes that constitute life? The answer lies in the molecular components that cooperate through a series of carefully-regulated processes to bring the information in our genome to life. These components and processes lie at the heart of one of the most fascinating subjects to engage the minds of scientists today: molecular biology.Molecular Biology: Principles of Genome Function, Second Edition, offers a fresh approach to the teaching of molecular biology by focusing on the commonalities that exist between the three kingdoms of life, and discussing the differences between the three kingdoms to offer instructive insights into molecular processes and components. This gives students an accurate depiction of our current understanding of the conserved nature of molecular biology, and the differences that underpin biological diversity. Additionally, an integrated approach demonstrates how certain molecular phenomena have diverse impacts on genome function by presenting them as themes that recur throughout the book, rather than as artificially separated topicsAs an experimental science, molecular biology requires an appreciation for the approaches taken to yield the information from which concepts and principles are deduced. Experimental Approach panels throughout the text describe research that has been particularly valuable in elucidating difference aspects of molecular biology. Each panel is carefully cross-referenced to the discussion of key molecular biology tools and techniques, which are presented in a dedicated chapter at the end of the book. Molecular Biology further enriches the learning experience with full-color artwork, end-of-chapter questions and summaries, suggested further readings grouped by topic, and an extensive glossary of key terms.Features: A focus on the underlying principles of molecular biology equips students with a robust conceptual framework on which to build their knowledgeAn emphasis on their commonalities reflects the processes and components that exist between bacteria, archae, and eukaryotesExperimental Approach panels demonstrate the importance of experimental evidence by describing research that has been particularly valuable in the field"
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The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis.
Book DescriptionMolecular Cloning has served as the foundation of technical expertise in labs worldwide for 30years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition,by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMIinvestigator Michael Green, preserves the highly praised detail and clarity of previous editionsand includes specific chapters and protocols commissioned for the book from expert practitionersat Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory,Washington University, and other leading institutions. The theoretical and historicalunderpinnings of techniques are prominent features of the presentation throughout, informationthat does much to help trouble-shoot experimental problems. For the fourth edition of this classicwork, the content has been entirely recast to include nucleic-acid based methods selected as themost widely used and valuable in molecular and cellular biology laboratories. Core chapters fromthe third edition have been revised to feature current strategies and approaches to the preparationand cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, andmanipulated, and how data generation and analysis can be handled. The new content includesmethods for studying interactions between cellular components, such as microarrays, nextgenerationsequencing technologies, RNA interference, and epigenetic analysis using DNAmethylation techniques and chromatin immunoprecipitation. To make sense of the wealth of dataproduced by these techniques, a bioinformatics chapter describes the use of analytical tools forcomparing sequences of genes and proteins and identifying common expression patterns amongsets of genes. Please take a look at our book preview site at molecularcloning.com. Building onthirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the newgold standard--the one indispensable molecular biology laboratory manual and reference source. 7ad9723583
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A descoberta da estrutura do DNA em 1953 representa um dos maiores avanços científicos da biologia. James D. Watson, um dos cientistas responsáveis pela descoberta, foi posteriormente reconhecido com o Prêmio Nobel por esta notável contribuição à ciência. Outra importante contribuição de Watson foi este livro, Biologia molecular do gene, que apresenta não apenas as bases intelectuais que ajudaram a moldar a biologia molecular no século passado, mas também toda a evolução do conhecimento alcançada desde então. 7ad9723583
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In order to determine the rate of evolution of the rGII.3, GII.3, GII.4 and GII.7 capsids, the nucleotide sequences of ORF2 were analysed. RNA capsid sequences used for the analysis included eight from this study and 76 sequences from GenBank, with the oldest strains available dating back to 1987. The strains used and their GenBank accession numbers are listed in Text S1. The rate of evolution (substitutions/nucleotide site/year) for GII.3, GII.b/GII.3 GII.4 and GII.7 NoVs was determined by calculating the number of nucleotide substitutions in ORF2 compared to an ancestral strain and this was plotted against time [27]. The rate of evolution was determined by linear regression with the program GraphPad PRISM® version 4 and was equivalent to the gradient of the line. Pairwise alignments of RNA sequences and evolutionary distances between sequences were carried out using the Maximum Composite Likelihood model in Mega 4.0 [28]. Bootstrapped trees (1000 data sets) were constructed using the Neighbour-joining method, also with the program Mega 4.0. 7ad9723583

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